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@#[Abstract] Objective: To investigate the effect of long non coding RNA (lncRNA) LINC00308 on proliferation, invasion and migration of prostate cancer cells and its related mechanism. Methods: lncRNAs and mRNAs differentially expressed in prostate cancer tissues and adjacent control tissues were screened by gene chip, and LINC00308 and TRIP13 (thyroid hormone receptor interactor13) were identified as the research objects. The effects of LINC00308 on the proliferation, invasion and migration of prostate cancer cells were detected by MTT assay, plate cloning, Transwell and scratch test. The above effects were verified in nude mice xenografts. The effect of LINC00308 on expression of TRIP13 in tumor tissues and cancer cells was detected by Western blotting and immunohistochemistry. Bioinformatics analysis, RIP (RNA immunoprecipitation), qPCR and Double luciferase gene reporter experiments were used to predict and explore the interaction mechanism between miR-361-5p and LINC00308 as well as TRIP13, and plate cloning and Transwell invasion test were used to verify the biological behaviors of cancer cells. Results: Both the microarray results and qPCR confirmed that the expressions of LINC00308 (P<0.01) and TRIP13 (P<0.05) were abnormally high in prostate cancer tissues and four cell lines; cell function test results showed that overexpression of LINC00308 could promote the proliferation, invasion and migration of prostate cancer PC3 cells (all P<0.05), while down-regulation of LINC00308 in prostate cancer cells had the opposite effect. In nude mice. LINC00308 could promote the tumorigenesis of prostate cancer cells in vivo, and increase the expression of TRIP13 both in vivo and in vitro (P<0.05). Bioinformatics analysis, RIP, qPCR and Double luciferase gene reporter results confirmed that miR-361-5p could bind to 3'-UTR of LINC00308 and TRIP13 respectively, and LINC00308 could act as a competing endogenous RNA (ceRNA) by sponging miR-361-5p to regulate the expression of TRIP13. In addition, MTT, plate cloning and Transwell assay confirmed the regulatory interaction among LINC00308 miR-361-5p and TRIP13 from the levels of proliferation, colony formation and invasion in cancer cells. Conclusion: LINC00308, which is abnormally highly expressed in prostate cancer tissues and cells, can inhibit the expression of miR-361-5p and enhance the expression of TRIP13 by exerting its ceRNA function, thus promoting the proliferation, invasion and migration of prostate cancer.
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Objective To investigate the clinical value of serum metabolomic profile of prostate cancer using nuclear magnetic resonance-based metabolomics.Methods The retrospective case control study was adopted.The clinical data of 31 patients with prostate cancer,28 patients of prostatic hyperplasia and 31 healthy volunteers were enrolled in this study from May 2016 to May 2017 at the first affiliated hospital of Xinjiang medical university.In PCa group,the mean age was 66.3 years old,ranging 53-80 years old.In BPH group,the mean age was 59.3 years old,ranging 46-75 years old.In volunteer group,the mean age was 47.8 years old,ranging 35-62 years old..The serum of the 3 groups was measured by 1H-NMR spectroscopy.Multivariate statistical analysis was used to analyze the serum differential metabolism of the 3 groups,including principal components analysis (PCA),partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA).Results The multivariate statistical analysis of PCA that the rate of the first principal component 1 (PC1) was 53.24％,the second principal component 2 (PC2) was 25.31％ and the cumulative contribution rate was 78.55 ％.Results of PLS -DA showed that partial data overlap of the three groups,but the separation trend was appeared.The variance of X(R2X) and Y(R2Y) matrixes and predictive value Q2 were 0.67,0.60,and 0.42.The results of OPLS-DA showed that the difference among the PCa group and BPH group,healthy group were obvious.The separation trend were appeared and the differential metabolites could be screened effectively.The R2X、R2Y and Q2 was 0.24,0.57,0.21 and 0.30,0.65,0.36.26 different serum metabolites were detected in the 3 groups,including citric acid,arginine,threonine,citrulline,glutamine,lactic acid,alanine,unsaturated fats,glycoprotein etc.Conclusions Compared with BPH group and healthy group,the serum of prostate cancer patients showed significant differences in metabolism.Nuclear magnetic resonance metabolomics analysis can effectively distinguish these serum metabolic differences.
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Objective To explore the epidemic situation of animal plague in Junggar Basin natural plague foci.Methods Data on epidemics of plague and on population involved,as well as results on antibodies and pathogens,were analyzed.Samples on animals and vectors were collected from 18 counties in Junggar Basin plague natural foci between 2007 and 2016.Results The density of Rhombomys (R.) opimus was temporally fluctuant,from 2.1/hm2 to 22.6/hm2 respectively.However,the spatial distribution appeared asymmetrical,with the highest seen in Kelamayi and Wumuqimidong counties,as 14.2/hm2 and 13.0/hrn2 respectively.Rates of capture on nocturnal rodents were from 4.2％ to 10.1％,with the highest rate as 10.1％ in 2014.Meriones meridianus appeared the dominant species in the nocturnal community of rodents,which accounted for 81.9％.Regarding the spatial and temporal distributions,rates ofR.opimus with fleas appeared fluctuant,with an average rate as 90.7％ and the average total flea index was 10.44.In flea community of R.opimus,Xenopsylla (X.)skrjabini was found the dominant species,popular in distribution and accounted for 47.8％.The average rate of nocturnal rodents with flea was 20.2％,with total flea index as 1.20 and the dominant fleas were X.conformis conformis and Nosopsyllus laeviceps.A total of 13 species with 9 087 serum samples from rodents were detected as having Y.pestis antibody by IHA,with 617 positive samples.Of them,the positive rate of having R.opimus appeared the highest (9.4％),followed by D.sagitta (1.1％).Spatially,two clustered areas were found,with one in the eastern Junggar Basin from Changji to Mulei county,with the antibody positive rates ofR.opimus as 14.3％.The other one was in the central area of Junggar Basin,including Kelamayi,Shawan and Wusu counties,with the antibody positive rate as 13.6％.The prevalence of plague on R.opimus was fluctuant,with the lowest seen in 2008,with the average antibody positive rate of R.opimus as 1.0％ and the highest as 19.3％ in 2013.A total of 18 strains were isolated from 2007 to 2016.However,most of the strains were isolated from R.opimus and parasitic fleas,accounted for 8/9,in Kelamayi,Wulumuqi-midong and Jimusaer,respectively.Conclusions Complex ecosystem was seen in the Junggar Basin natural plague foci,with the multiple composition of species and different community structure of hosts and vectors,plus the flustering prevalence.Animal plague was seen in the whole region with succession of the plague nature foci.Passive transmission of plague between human beings and animals through close contacts was seen which was driven by economic benefits to some degree.
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Objective To explore the epidemic situation of animal plague in Junggar Basin natural plague foci.Methods Data on epidemics of plague and on population involved,as well as results on antibodies and pathogens,were analyzed.Samples on animals and vectors were collected from 18 counties in Junggar Basin plague natural foci between 2007 and 2016.Results The density of Rhombomys (R.) opimus was temporally fluctuant,from 2.1/hm2 to 22.6/hm2 respectively.However,the spatial distribution appeared asymmetrical,with the highest seen in Kelamayi and Wumuqimidong counties,as 14.2/hm2 and 13.0/hrn2 respectively.Rates of capture on nocturnal rodents were from 4.2％ to 10.1％,with the highest rate as 10.1％ in 2014.Meriones meridianus appeared the dominant species in the nocturnal community of rodents,which accounted for 81.9％.Regarding the spatial and temporal distributions,rates ofR.opimus with fleas appeared fluctuant,with an average rate as 90.7％ and the average total flea index was 10.44.In flea community of R.opimus,Xenopsylla (X.)skrjabini was found the dominant species,popular in distribution and accounted for 47.8％.The average rate of nocturnal rodents with flea was 20.2％,with total flea index as 1.20 and the dominant fleas were X.conformis conformis and Nosopsyllus laeviceps.A total of 13 species with 9 087 serum samples from rodents were detected as having Y.pestis antibody by IHA,with 617 positive samples.Of them,the positive rate of having R.opimus appeared the highest (9.4％),followed by D.sagitta (1.1％).Spatially,two clustered areas were found,with one in the eastern Junggar Basin from Changji to Mulei county,with the antibody positive rates ofR.opimus as 14.3％.The other one was in the central area of Junggar Basin,including Kelamayi,Shawan and Wusu counties,with the antibody positive rate as 13.6％.The prevalence of plague on R.opimus was fluctuant,with the lowest seen in 2008,with the average antibody positive rate of R.opimus as 1.0％ and the highest as 19.3％ in 2013.A total of 18 strains were isolated from 2007 to 2016.However,most of the strains were isolated from R.opimus and parasitic fleas,accounted for 8/9,in Kelamayi,Wulumuqi-midong and Jimusaer,respectively.Conclusions Complex ecosystem was seen in the Junggar Basin natural plague foci,with the multiple composition of species and different community structure of hosts and vectors,plus the flustering prevalence.Animal plague was seen in the whole region with succession of the plague nature foci.Passive transmission of plague between human beings and animals through close contacts was seen which was driven by economic benefits to some degree.
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OBJECTIVE: Using a pulsating coronary artery phantom at high heart rate settings, we investigated the efficacy of a motion correction algorithm (MCA) to improve the image quality in dual-energy spectral coronary CT angiography (CCTA). MATERIALS AND METHODS: Coronary flow phantoms were scanned at heart rates of 60–100 beats/min at 10-beats/min increments, using dual-energy spectral CT mode. Virtual monochromatic images were reconstructed from 50 to 90 keV at 10-keV increments. Two blinded observers assessed image quality using a 4-point Likert Scale (1 = non-diagnostic, 4 = excellent) and the fraction of interpretable segments using MCA versus conventional algorithm (CA). Comparison of variables was performed with the Wilcoxon rank sum test and McNemar test. RESULTS: At heart rates of 70, 80, 90, and 100 beats/min, images with MCA were rated as higher image scores compared to those with CA on monochromatic levels of 50, 60, and 70 keV (each p < 0.05). Meanwhile, at a heart rate of 90 beats/min, image interpretability was improved by MCA at a monochromatic level of 60 keV (p < 0.05) and 70 keV (p < 0.05). At a heart rate of 100 beats/min, image interpretability was improved by MCA at monochromatic levels of 50 keV (from 69.4% to 86.1%, p < 0.05), 60 keV (from 55.6% to 83.3%, p < 0.05) and 70 keV (from 33.3% to 69.3%, p < 0.05). CONCLUSION: Low-keV monochromatic images combined with MCA improves image quality and image interpretability in CCTAs at high heart rates.
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Angiography , Coronary Vessels , Heart Rate , Heart , Tomography, X-Ray ComputedABSTRACT
Objective To introduce a new trocar position in the posterior laparoscopic for the treatment of retrocaval ureter.Methods From August 2011 to October 2014,5 cases with retrocaval ureter treated with posterior laparoscopic were retrospectively analyzed,including 3 males and 2 females,aged from 15 to 46 years(mean 34 years).The history of disease ranged from 1 to 10 months,with 3 cases presented with low back pain,and 2 cases being detected uronephrosis by check-up.Results All the operations were successfully completed,with the operation duration ranged from 75-125 min (mean 90min),and blood loss ranged from 20ml to 50 ml(mean 35 ml).The average hospital stay was 6 days(5-7d).There was no wound infection or urine leakage.Ureteral double-J tubes were removed 4 weeks after surgery.Postoperative followup ranged from 2 weeks to 6 months (mean 30 months).There was no anastomotic stricture,and the hydronephrosis relieved.Conclusions Trocar position adjustment of posterior laparoscopic in treatment of retrocaval ureter is convenient to operate,which also shortened the time of operation,reduced the difficulty of operation and the surgeons' fatigue.
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<p><b>OBJECTIVE</b>To understand the distribution, fauna, population structure of host animals and their parasitic fleas as well as popular dynamic of animal plague of natural plague foci in Junggar Basin.</p><p><b>METHODS</b>Sample materials and data of animals and vector insects were collected using ecological methods and the population structures were analyzed statistically. F1 antibody of Yersinia pestis in rodents' serum and organ suspension was detected by means of IHA while the pathogen of Y. pestis in rodents and vector insects was detected by means of aetiological detections and the isolated Y. pestis was detected using biochemical methods.</p><p><b>RESULTS</b>The small mammals which were found in Junggar Basin belonged to 17 species of 11 genera 7 families. Of them, 13 species of rodents were included whose parasitic fleas belonged to 19 species of 10 genera 8 families. The average coverage of Rhombomys opimus hole-community was 22.5% in Junggar Basin with the average density of R. opimus hole-community was 15.9/hm2 and the average rate of habitat of the hole-community was 70.2%. In the R. opimus community, the average density of rodents was 3.1/hole-community, and 34.4/hm2 in the nature plague foci. In the population structure of the hole-community of R. opimus, R. opimus accounted for 72.9% in the total captured rodents, Meriones meridianus was 24.5% while the others were 2.6%. In the nocturnal community of rodents, M. meridianus accounted for 64.0% in total captured rodents, Dipus sagitta was 15.1%, M. erythrourns was 7.5% and the others were 13.4%. In the rodents community of Junggar Basin, the rate of R. opimus with fleas was 84.9%, which was the highest, followed by M. tamariscinus, Euchoreutes naso and M. erythrourns, with the rates as 71.4%, 66.7% and 62.7% respectively. The rate of M. meridianus with fleas was 38.3%. There were 16 species of parasitic fleas in R. opimus, with the total flea index as 8.58 and the dominant species was Xenopsylla skrjabini. There were 17 and 16 kinds of fleas in M. erythrourns and M. meridianus respectively with the total flea index were 1.59 and 1.15, with dominant fleas were Nosopsyllus laeviceps and X. skrjabini. The serum and organ suspension of 3179 rodents which belonged to 12 species were detected by means of IHA, of them 174 samples were positive and the positive rate was 5.5%. There were 1356 samples of R. opimus in these materials, and 164 were positive, accounted for 12.1%. The samples of M. meridianus were 1255, with 9 positive, accounted for 0.7%. The samples of D. sagitta were 116 with 1 positive and the rate was 0.9%. The samples of other rodents were 452 but were all negative. There were in total 2975 organs collected from rodents, when detected by methods of isolated of Y. pestis. 15 strains of Y. pestis were isolated from 1243 R. opimus, and 2 strains isolated from 1230 M. meridianus. A total number of 11 647 fleas from rodents were detected by methods of isolated of Y. pestis in which 1 strain of Y. pestis was isolated from 4713 X. skrjabini, and 6 were isolated from 2101 Xenopsylla minax, 1 from 328 Xenopsylla conformis conformis and 1 from 250 Echidnophaga oschanini. Among the other 4255 fleas, none was isolated. The biochemical properties of these Y. pestis which isolated from Junggar Basin were positive of Maltose, Ejiao sugar and Glycerol, and negative of Rhamnose and Nitrogen, which were all strongly poisonous to mouse.</p><p><b>CONCLUSION</b>The natural plague foci in Junggar Basin spread all over the whole Junggar Basin. There were animal plague cases found in 12 counties (cites) while Karamy, Bole, Jimusaer and Qitai were confirmed as plague foci counties (cities). Animals and vector insects of the foci were complicated but the ecological system was stable. R. opimus was recognized as the dominant host animal and its biochemical type belonged to the Middle Ages, suggesting that the foci was a new type of natural plague foci.</p>
Subject(s)
Animals , Mice , China , Epidemiology , Gerbillinae , Microbiology , Plague , Epidemiology , Microbiology , Rodent Diseases , Epidemiology , Microbiology , Yersinia pestis , Allergy and Immunology , VirulenceABSTRACT
<p><b>OBJECTIVE</b>To compare the molecular characteristics of 3 Crimean-Congo hemorrhagic fever viruses(CCHFV) isolated in Xinjiang province.</p><p><b>METHODS</b>YL05035, YT05099 and LT05146 were isolated in 2005 from Hyalomma ticks and viral RNA was extracted from suckling mouse brains infected with these three strains respectively. The polymerase chain reaction(PCR) products of S segments from the 3 strains of CCHFV were directly sequenced.</p><p><b>RESULTS</b>The full-length'S RNA from the 3 strains of CCHFV all comprised 1673 nucleotides with ORF of them including 1449 nucleotides and encoding a protein which comprised 482 amino acids in a viral complementary sense. The sequences indicated that the three strains of CCHFV isolated from ticks in Xinjiang province were highly homologenic. Data from the phylogenetic analysis showed that the obtained sequences were identical. The homology between 3 strains of CCHFV was 99.5%. Their homologies compared with that of the other strains isolated from other region of Xinjiang were also high at nucleotide levels (92.7%-99.8%). The three strains which were clustered together with 7001 strain and 79121 strain (isolated from patient and rat in Xinjiang respectively) was only different by 2%-3%. The genetic difference from the prototype CCHFV Nigerian strain IBAR10200 was 13%. In comparison, the Nigerian CCHFV tick isolate was more divergent when compared with the reference China strains 66019 and with the three variants mentioned above.</p><p><b>CONCLUSION</b>The CCHFV isolated from China comprised a group of genetically high conserved strains.</p>